Les Biller was born in Los Angeles, received his B.A. from the University of Hawaii in 1957 and his MFA from UCLA in 1960. In the 1960s through the 1980s he exhibited regularly in galleries in both Honolulu and Los Angeles. He has had one-artist exhibitions at the Frederick Wight Gallery, UCLA; the Contemporary Art Center in Honolulu; and the Contemporary Arts Forum, Santa Barbara. A large survey exhibition of Biller's work, curated by Michael Duncan, was held at the College of the Canyons in Santa Clarita, California, including a catalogue. He was awarded the Painting Prize in the Los Angeles County Museum of Art's Annual in 1959 and a Fullbright Grant, affiliated with Tokyo University of the Arts, Tokyo, 1960-62.

Biller has held teaching positions at UCLA and the University of Hawaii, Honolulu. He has exhibited with the Rosamund Felsen Gallery since 2014.